What question did this study set out to answer?

Investigate the interaction of carbon dioxide and bicarbonate with fluorine-labeled amino acids using fluorine-19 nuclear magnetic resonance spectroscopy.

October 1, 1985Canadian Journal of Biochemistry and Cell Biology

A 19F nuclear magnetic resonance study of the interaction of carbon dioxide with fluoro-amino acids

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Investigate the interaction of carbon dioxide and bicarbonate with fluorine-labeled amino acids using fluorine-19 nuclear magnetic resonance spectroscopy.
Analyzed 19F nuclear magnetic resonance spectra of 3-fluorophenylalanine and 3-fluorotyrosine across varying pH (pD) levels in bicarbonate buffer solutions.
Quantified free amino acid and carbamate concentrations from normalized spectral peak intensities using an equation accounting for the ionic states of reactants.
Determined carbamate reaction equilibrium constants of 8.52 × 10⁻⁶ ± 0.27 × 10⁻⁶ for 3-fluorophenylalanine and 8.84 × 10⁻⁶ ± 0.72 × 10⁻⁶ for 3-fluorotyrosine.
Identified a well-resolved upfield resonance corresponding to carbamate formation, demonstrating that fluorine substitution does not significantly perturb the reaction.

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Implication

In vitro spectroscopic analysis demonstrates reversible carbamate formation with carbon dioxide in fluoro-amino acids, indicating fluorine-19 NMR reliably quantifies carbon dioxide binding.

Cite This Study

Dettman et al. (1985) studied this question.

synapsesocial.com/papers/6aa8471ebe5ad42562269f34 https://doi.org/10.1139/o85-140

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Implication

In vitro spectroscopic analysis demonstrates reversible carbamate formation with carbon dioxide in fluoro-amino acids, indicating fluorine-19 NMR reliably quantifies carbon dioxide binding.

A 19F nuclear magnetic resonance study of the interaction of carbon dioxide with fluoro-amino acids

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Discussion

Implication

Cite This Study

A 19F nuclear magnetic resonance study of the interaction of carbon dioxide with fluoro-amino acids

Key Points

Discussion

Implication

Cite This Study

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