Anticonvulsant activity and neuroprotection assay of 3-substituted-N-aryl-6,7-dimethoxy-3a,4-dihydro-3H-indeno[1,2-c]pyrazole-2-carboxamide analogues

In the present investigation we report herein the synthesis and neuroprotection assay of 3-substituted- N -aryl-6,7-dimethoxy-3a,4-dihydro-3 H -indeno[1,2-c]pyrazole-2-carboxamide ( 4a – c ). The anticonvulsant activity and neuroprotection assay were done according to Antiepileptic Drug Development Programme (ADD) protocol reported elsewhere. The compounds showed significant anticonvulsant and neuroprotective activities. The compound 4b showed neuroprotection activity with 26.2 ± 1.9% of total propidium iodide uptake at 100 μM and IC 50 of the compound was calculated using dose response curve by probit analysis and was found to be 159.20 ± 1.21 μM.