Glycine Specific [3h]strychnine Binding in the Pigeon CNS

Glycine sensitive [3H]strychnine binding has been demonstrated in the pigeon CNS. The kinetic parameters in spinal cord, tectum, telencephalon and cerebellum in this order were: Kn, (nM) 5, 5, 2.3, 6 and Bmax (fmol/mg protein) 1000, 275, 92, 20. The effect of various amino acids and strychnine analogs on the binding was measured. Retinal ablation caused a 22% decrease in the glycine sensitive [3H]strychnine binding in the tectum while lesions destroying up to 80% of the nucleus Ipc had no effect.