Validation of a Suitable Housekeeping Gene in Human Neuroblastoma SHSY-5Y Cell-Line Treated with Steroid Hormones Progesterone and Testosterone

In this study, we evaluated the stability of three common housekeeping genes, GAPDH, beta-Actin, and 18S-rRNA, in human neuroblastoma SHSY-5Y cells exposed to varying levels of steroid hormones like progesterone (P4) and testosterone. Housekeeping genes serve as reliable internal controls, assumed to maintain consistent expression across diverse tissues and experimental conditions. Since steroid hormones act as ligand-activated transcription factors, directly impacting gene transcription, the identification of suitable housekeeping genes in steroid hormone-influenced tissues is imperative.