What question did this study set out to answer?

Develop and validate rapid, extraction-free RT-qPCR and RT-LAMP saliva assays to facilitate mass monitoring and screening of SARS-CoV-2.

October 31, 2021MoleculesOpen Access

Robust Saliva-Based RNA Extraction-Free One-Step Nucleic Acid Amplification Test for Mass SARS-CoV-2 Monitoring

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Authors

EREva Rajh TŠTina Šket APArne PraznikUniversity of Ljubljana

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Implication

Diagnostic evaluation demonstrates high sensitivity of extraction-free saliva RT-qPCR in symptomatic and asymptomatic individuals, indicating viable mass screening without nasal swabs.

Key Points

Develop and validate rapid, extraction-free RT-qPCR and RT-LAMP saliva assays to facilitate mass monitoring and screening of SARS-CoV-2.
Evaluated over 700 matched pairs of saliva (passive drool or oral cavity swab) and nasopharyngeal swab specimens collected from symptomatic and asymptomatic individuals.
Stabilized saliva samples in an RNA buffer, applied heat treatment, and conducted nucleic acid amplification directly without RNA extraction.
Compared diagnostic sensitivity of extraction-free saliva RT-qPCR and RT-LAMP against standard clinical RT-qPCR performed on nasopharyngeal swabs.
Saliva-based RT-qPCR demonstrated at least 95% diagnostic sensitivity in individuals with subclinical infection compared to nasopharyngeal clinical RT-qPCR, whereas RT-LAMP demonstrated at least 70% sensitivity.
Passive drool saliva achieved a higher diagnostic sensitivity than oral cavity swab specimens (95% vs. 87%).

Cite This Study

Rajh et al. (2021) studied this question.

synapsesocial.com/papers/6aa86a2ab0bbeaafc8aa928f https://doi.org/10.3390/molecules26216617

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Implication

Diagnostic evaluation demonstrates high sensitivity of extraction-free saliva RT-qPCR in symptomatic and asymptomatic individuals, indicating viable mass screening without nasal swabs.

Robust Saliva-Based RNA Extraction-Free One-Step Nucleic Acid Amplification Test for Mass SARS-CoV-2 Monitoring

Discussion

Member takes

Implication

Key Points

Cite This Study

Robust Saliva-Based RNA Extraction-Free One-Step Nucleic Acid Amplification Test for Mass SARS-CoV-2 Monitoring

Discussion

Member takes

Implication

Key Points

Cite This Study

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