Key result
ELISA-I detects classical swine fever virus antibodies with ~98% sensitivity versus standard NPLA.
Why the study?
An indirect ELISA was developed and standardized for serological diagnosis of classical swine fever to provide a suitable large-scale screening method.
Does an indirect ELISA-I accurately diagnose classical swine fever compared to NPLA in swine serum samples?
Does an indirect ELISA-I accurately diagnose classical swine fever compared to NPLA in swine serum samples?
The developed indirect ELISA is a highly sensitive and specific tool suitable for large-scale screening of classical swine fever virus antibodies, though it cross-reacts with other pestiviruses.
ELISA-I may aid CSFV screening in swine; leaves open field validation and cross-reactivity assessment.
An indirect enzyme linked immunoassay (ELISA-I) was developed and standardized for the serological diagnosis of classical swine fever (CSF). For the comparison, nine hundred and thirty-seven swine serum samples were tested by serum neutralization followed by immunoperoxidase staining (NPLA), considered as the standard. Of these, 223 were positive and 714 negative for neutralizing antibodies to classical swine fever virus (CSFV). In relation to the NPLA, the ELISA-I presented a 98.2% sensitivity; 92.86% specificity, 81.11% positive predictive value, 99.4% negative predictive value and a 94.1% precision. Statistical analysis showed a very strong correlation (r=0,94) between both tests. When compared to a commercially available ELISA kit, the performance of both, in relation to the NPLA, was similar. It was concluded that the ELISA-I is suitable for large scale screening of antibodies to classical swine fever virus, although it does not distinguish antibodies to classical swine fever virus from those induced by other pestiviruses.
No takes yet. Share an insight, caveat, or question.
Paredes et al. (1999) studied Classical swine fever (n=937). Indirect enzyme linked immunoassay (ELISA-I) vs. Serum neutralization followed by immunoperoxidase staining (NPLA) was evaluated on Diagnostic sensitivity and specificity. The developed ELISA-I demonstrated 98.2% sensitivity and 92.86% specificity for detecting classical swine fever virus antibodies compared to the standard NPLA test.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: