Key result
The immunochromatographic strip test detected anti-DEV antibodies in 37.27% of samples, showing sensitivity comparable to ELISA and higher than NT.
Why the study?
Monitoring DEV-specific antibodies is essential to evaluate DEV vaccine efficacy and develop immunization programs, but rapid, specific, and sensitive detection methods are needed.
Does an immunochromatographic strip test based on recombinant UL51 protein accurately detect DEV serum antibodies compared to ELISA and NT in duck serum samples?
Does an immunochromatographic strip test based on recombinant UL51 protein accurately detect DEV serum antibodies compared to ELISA and NT in duck serum samples?
A newly developed immunochromatographic strip test provides a rapid, specific, and sensitive method for detecting duck enteritis virus antibodies without requiring specialized equipment.
Answers are grounded in this paper and cite their sources.
May enable rapid DEV antibody detection in ducks without equipment; leaves open prospective validation before wider adoption.
BACKGROUND: Duck enteritis virus (DEV) infection causes substantial economic losses to the worldwide duck-producing areas. The monitoring of DEV-specific antibodies is a key to evaluate the effect of DEV vaccine and develop rational immunization programs. Thus, in this study, an immunochromatographic strip (ICS) test was developed for detecting DEV serum antibodies. RESULTS: The ICS test is based on membrane chromatography, and uses both the purified recombinant UL51 protein conjugated with colloidal gold and goat anti-rabbit IgG conjugated with colloidal gold as tracers, the purified recombinant UL51 protein as the capture reagent at the test line, and rabbit IgG as the capture reagent at the control line. The specificity of the ICS was evaluated by sera against DEV, Duck hepatitis virus (DHV), Riemerella anatipestifer (RA), Duck E. coli, Muscovy duck parvovirus (MPV), or Duck Influenza viruses (DIV). Only sera against DEV showed the strong positive results. In order to determine the sensitivity of the ICS, anti-DEV serum diluted serially was tested, and the minimum detection limit of 1:128 was obtained. The ICS components, which are provided in a sealed package, require no refrigeration and are stable for 12 months. To evaluate the effect of the ICS, 110 duck serum samples collected from several non-immune duck flocks were simultaneously tested by the ICS test, enzyme-linked immunosorbent assay (ELISA) and neutralization test (NT). The results showed that the sensitivity of the ICS test was almost consistent with ELISA and much higher than NT, has low cost, and is rapid (15 min) and easy to perform with no requirement of specialized equipment, reagent or technicians. CONCLUSIONS: In this work, we successfully developed a simple and rapid ICS test for detecting DEV serum antibodies for the first time. The ICS test was high specific and sensitive for the rapid detection of anti-DEV antibodies, and has great potential to be used for the serological surveillance of DEV infection in the field.
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Shen et al. (2010) studied Duck enteritis virus (DEV) infection (n=110). Immunochromatographic strip (ICS) test vs. ELISA and Neutralization Test (NT) was evaluated on Detection rate of anti-DEV positive sera (p=≥0.05). The immunochromatographic strip test detected anti-DEV antibodies in 37.27% of samples, showing sensitivity comparable to ELISA and higher than NT.
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