Key result
Sequencing the sheep myostatin gene promoter reveals multiple conserved muscle growth response elements.
Why the study?
The structure and function of the myostatin gene promoter region in sheep were not fully understood.
The study identified the structure and regulatory elements of the sheep myostatin promoter, providing a basis for understanding its function in muscle growth.
Answers are grounded in this paper and cite their sources.
Supports targeted mutagenesis of ovine myostatin elements; leaves open translation to human muscle disorders or therapies.
To better understand the structure and function of the myostatin's gene promoter region in sheep, we cloned and sequenced a 1.517 kb fragment containing the 5'-regulatory region of the sheep myostatin gene (GenBank accession number is AY918121). The promoter sequence consists of three TATA boxes, one CAAT box, and eight putative E-boxes. Some putative muscle growth response elements for Octamer-binding factor 1(Octamer), Activator protein 1(AP1), Growth factor independence 1 zinc finger protein (Gfi-1B), Myocyte enhancer factor 2 (MEF2), Muscle-specific Mt binding site (MTBF), Glucocorticoid response elements (GRE) and Progesterone receptor binding site (PRE) were detected. Some of the motifs are conserved as compared to with that in the goat, bovine and porcine myostatin promoters. However, some differences were also found.
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Du et al. (2005) studied this question. Cloning and sequence analysis of myostatin promoter was evaluated on Sequence analysis of the 5'-regulatory region of the sheep myostatin gene. Cloning and sequencing of a 1.517 kb fragment of the sheep myostatin gene promoter revealed three TATA boxes, one CAAT box, eight E-boxes, and several conserved muscle growth response elements.
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