Analytical study demonstrates precise quantification of 17-beta-estradiol in transdermal gels, highlighting an efficient microextraction technique for quality control.
A method is proposed for the chromatographic determination of 17-β-estradiol in medicinal preparations with the microextraction of the analyte from a sample solution. The procedure involves dispersing the extractant (1-dodecanol) in the aqueous phase by rotating a cotton disk with a magnetic stirrer insert. The formation of the extractant emulsion ensures the rapid mass transfer of the target analyte. When the disk rotates in the aqueous phase, a central vortex also forms, in which a drop of the extract is formed with the subsequent destruction of the emulsion. Cooling the extraction system allows the crystallization of the extract, which is taken for an HPLC-UV analysis. The limit of detection (3σ) of 0.03 mg/L is reached. The analytical capabilities of the developed method are shown on an example of the determination of 17-β-estradiol in transdermal gels.
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Yakupova et al. (2022) studied this question.
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