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Microfluidics-based lithography based on microfluidics is used to pattern cell-adhesive hydrogel substrates with micro-variations in stiffness. The micropatterns are generated by feeding PEG-fibrinogen and various amounts of polyethylene glycol diacrylate (PEGDA) into the micofluidic channel (see picture).
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Cheung et al. (2009) studied this question.
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