TaPR1-66 functions as a positive regulator of wheat stripe rust resistance

Wheat stripe rust is a devastating disease caused by Puccinia striiformis f. sp. tritici ( Pst ), which seriously threatens the safety of wheat production in China. The pathogenesis-related protein-1 (PR1) gene is important for plants to respond to various biotic and abiotic stresses. In this study, TaPR1-66 was selected as a candidate gene based on previous gene family identification and transcriptome sequencing analysis. Virus-induced gene silencing indicated that TaPR1-66 positively regulated wheat stripe rust resistance. Subcellular mapping indicated that TaPR1-66 was located in the periphery. This study preliminarily verified that TaPR1-66 positively regulated wheat XC32 resistance to stripe rust, which provided a theoretical basis for the mining of resistance genes and the creation of new germplasm materials.