CTLA-4 (+49A/G) Polymorphism and Type-1 Diabetes in Turkish Children

© Jo ur nal of Cli ni cal Re se arch in Pe di at ric En doc ri no logy, Pub lis hed by Ga le nos Pub lis hing. CTLA-4 (+49A/G) Polymorphism and Type-1 Diabetes in Turkish Children ABS TRACT Ob jec ti ve:To evaluate the contribution of cytotoxic T-Iymphocyte antigen-4 (CTLA-4)+49A/G polymorphism to the susceptibility to type-1 diabetes (T1D) in Turkish children. Methods:A case-control study was designed to include 91 Turkish children with T1D and 99 healthy controls.CTLA-4 (+99A/G) gene polymorphism typing was done by PCR amplification followed by restriction fragment length polymorphism method.Results: The genotype and allele frequencies of the CTLA-4 (+99A/G) polymorphism in patients with T1D were not different from those in the controls (p>0.05).The allele frequency of G was 36.2% in patients with T1D, and 31.8% in controls (p>0.05).Additionally, this polymorphism was not associated with the clinical and laboratory characteristics of the patients with T1D (p>0.05).Conclusions: Our case-control study suggests that the CTLA-4 (+99A/G) gene polymorphism is not associated with T1D in the Turkish population.