What question did this study set out to answer?

To determine whether transgenic expression of HLA-G on porcine endothelial cells blocks human natural killer cell adhesion and subsequent xenogeneic cytotoxicity.

November 1, 2001The Journal of Immunology

HLA-G Inhibits Rolling Adhesion of Activated Human NK Cells on Porcine Endothelial Cells

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Authors

PFPietro ForteIstituto Nazionale Tumori IRCCS "Fondazione G. Pascale"LPLaszlo PazmanyHarvard University UMUlrike B. Matter‐ReissmannUniversity Hospital of Zurich

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Implication

In vitro study demonstrates that HLA-G expression inhibits human NK cell adhesion and cytotoxicity against pig endothelium, highlighting potential strategies to prevent xenograft rejection.

Key Points

To determine whether transgenic expression of HLA-G on porcine endothelial cells blocks human natural killer cell adhesion and subsequent xenogeneic cytotoxicity.
Stably transfected two immortalized porcine endothelial cell lines (2A2 and PED) with HLA-G1.
Assayed rolling adhesion of activated human NK cells and xenogeneic cytotoxicity using both polyclonal NK cell lines and cloned NK cells.
Used monoclonal antibodies targeting HLA-G and the inhibitory receptor ILT-2 to identify the receptor-ligand interactions involved.
HLA-G1 expression on porcine endothelial cells inhibited rolling adhesion of activated human NK cells and reduced cytotoxicity by polyclonal lines and NK clones.
Adhesion inhibition was partially reversed by antibody blockade of either HLA-G or the NK cell receptor ILT-2.
Cytotoxicity suppression by HLA-G was only partly dependent on ILT-2, indicating that additional inhibitory receptors are also involved.

Cite This Study

Forte et al. (2001) studied this question.

synapsesocial.com/papers/6aa8f95f1a422052082c8488 https://doi.org/10.4049/jimmunol.167.10.6002

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Implication

In vitro study demonstrates that HLA-G expression inhibits human NK cell adhesion and cytotoxicity against pig endothelium, highlighting potential strategies to prevent xenograft rejection.

HLA-G Inhibits Rolling Adhesion of Activated Human NK Cells on Porcine Endothelial Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

HLA-G Inhibits Rolling Adhesion of Activated Human NK Cells on Porcine Endothelial Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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