Answers are grounded in this paper and cite their sources.
An antiserum was produced in one rabbit against mouse zonae pellucidae solubilized with 70 mM Na2SO3, 1% SDS, and 0.04 mM CuSO4. An IgG of zona antibody completely inhibited fertilization both in vitro and in vivo in the mouse. F(ab′)2 fragment obtained by pepsin digestion of IgG zona antibody inhibited fertilizability of eggs in vitro but did not inhibit fertilization in vivo after passive immunization.
No takes yet. Share an insight, caveat, or question.
Tsunoda et al. (1982) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: