Effect of Hematopoietic Stem Cells and Platelet-Rich Plasma on the Healing of Experimental Skin Burned Tissues: A Comparative Study in Adult Male Mice

This is the authors' abstract. We don't add key points for this paper.

Background: Unfortunately burns are a common problem, leading to scarring or death. Platelet rich plasma (PRP) is a well known method that could be used as topical application for burn either second or third degree; it harbors many growth factors that can accelerate the healing. This Study Aimed: To analyze the use of PRP in deep second-degree burn, in comparison with the hematopoietic stem cells (CD34+) in mice. Materials and Methods: Seventy male adult mice were divided into four groups (control group, burn only, burn treated with CD34+ cells (injected once intradermal with 0.3–0.4 × 106 /kg and burn treated with PRP (1 ml injected once intradermal) at the edge of burn after 24hs from its induction. All burned groups were exposed to thermal burn. On day 9 and 19, the animals were sacrificed and skin biopsies were taken for H&E and Masson's trichrome staining. Assessment of angiogenesis and quantification study for matrix metalloproteinase 13 (MMP13) was done. Results: Wounds treated with PRP revealed fast wound closure in comparison with burned only group. Significant decrease in collagen fibers and increase in MMP13 deposition, as well as angiogenic markers (Angio1 and 2 and VEGF) expression was observed. However CD34+ cells treated group showed highly significant improvement in previously mentioned markers. Histopathological changes of burned skin improved in all treated groups after 19 days particularly in CD34 + cells treated group. Conclusion: These results indicated that CD34+ cells treatment could exert beneficial effects on healing process more than that of PRP in second degree burns.