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Develop and validate a rapid microwave-assisted extraction method to identify and quantify saccharides and polyols in Spirulina (Arthrospira platensis) grown under varying salinity conditions.

February 24, 2021Arabian Journal of ChemistryOpen Access

A microwave-based technique to determine saccharides and polyols contents in Spirulina (Arthrospira platensis)

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Authors

RFRohmah Nur Fathimah WSWidiastuti SetyaningsihUniversitas Gadjah Mada CCCeferino CarreraAgriculture and Agri-Food Canada

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Implication

Analytical study demonstrates microwave-assisted extraction quantifies saccharides and polyols in Spirulina, indicating salinity stress increases osmolytes six-fold.

Key Points

Develop and validate a rapid microwave-assisted extraction method to identify and quantify saccharides and polyols in Spirulina (Arthrospira platensis) grown under varying salinity conditions.
Optimized extraction conditions (temperature: 30–80 °C, ethanol solvent: 0–50%, and solvent-to-sample ratio: 10:1–30:1 mL/g) using a Box-Behnken design with Response Surface Methodology.
Conducted kinetic recovery studies and analytically validated the method for linearity, detection limits, quantification limits, precision, and accuracy.
Applied the optimized method to Spirulina biomass cultivated in media with salinity concentrations of 0, 3, 17, and 25 g L⁻¹ sodium chloride.
Optimal extraction conditions of 30 °C, 50% aqueous ethanol, and a 30:1 solvent-to-sample ratio yielded full analyte recovery within 15 minutes.
Resolved and quantified five primary target compounds: inositol, glycerol, sorbitol, glucose, and maltose.
Cultivation in high-salinity media (17 and 25 g L⁻¹ NaCl) produced a six-fold higher total sugar and polyol concentration than low-salinity media (0 and 3 g L⁻¹ NaCl), driven by substantial increases in glycerol and glucose.

Cite This Study

Fathimah et al. (2021) studied this question.

synapsesocial.com/papers/6aa904f939e8b1c7dca34409 https://doi.org/10.1016/j.arabjc.2021.103094

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Implication

Analytical study demonstrates microwave-assisted extraction quantifies saccharides and polyols in Spirulina, indicating salinity stress increases osmolytes six-fold.

A microwave-based technique to determine saccharides and polyols contents in Spirulina (Arthrospira platensis)

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Implication

Key Points

Cite This Study

A microwave-based technique to determine saccharides and polyols contents in Spirulina (Arthrospira platensis)

Discussion

Member takes

Implication

Key Points

Cite This Study

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