Key result
Blocking or deleting the Ang-1-7 Mas receptor in rodents disrupts spermatogenesis and reduces sperm production.
Why the study?
Does the genetic deletion or chronic blockage of Ang-(1-7) receptor Mas affect spermatogenesis and male fertility in mice and rats?
Does the genetic deletion or chronic blockage of Ang-(1-7) receptor Mas affect spermatogenesis and male fertility in mice and rats?
The Ang-(1-7) receptor Mas plays an important role in the regulation of spermatogenesis in mice and rats.
Mas receptor disruption impairs rodent spermatogenesis; hypothesis-generating for fertility effects of Ang-(1-7) pathway modulators in humans.
Evidence regarding the components of the renin-angiotensin (Ang) system suggests that this system plays an important role in male reproduction. However, there are few data available in the literature on the effects of Ang-(1-7) on the male reproductive system. The present study investigated the effects of the genetic deletion and chronic blockage of Ang-(1-7) receptor Mas on spermatogenesis and male fertility. The localization of Mas in mouse and rat testes was determined by binding assays and immunofluorescence, whereas the testis structure and spermatogenic process were morphologically and stereologically analysed by light microscopy. Ang-(1-7) binding and immunofluorescence revealed the presence of Mas in the testes of mice and rats. Although the total numbers of Sertoli and Leydig cells per testis and Leydig cell size were similar in both wild-type and Mas-deficient mice, Mas(-/-) animals exhibited a significant reduction in testis weight and a greater volume of apoptotic cells, giant cells and vacuoles in the seminiferous epithelium. In both mice and rats, an increased number of apoptotic cells were found during meiosis. Due to disturbed spermatogenesis, daily sperm production was markedly reduced in Mas(-/-) mice. Moreover, chronic infusion of A-779 [an Ang-(1-7) antagonist] in rats significantly increased the total number of apoptotic cells and primary spermatocytes in particular stages of spermatogenesis. Taken together, these findings strongly suggest that Ang-(1-7) receptor Mas plays an important role in the regulation of spermatogenesis.
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Leal et al. (2009) studied Spermatogenesis and male fertility. Genetic deletion of Mas receptor (Mas-/-) and chronic infusion of A-779 vs. Wild-type mice and control rats was evaluated on Spermatogenesis, testis structure, daily sperm production, and apoptotic cells. Genetic deletion or chronic blockade of the Ang-(1-7) receptor Mas in mice and rats resulted in disturbed spermatogenesis, reduced daily sperm production, and increased apoptotic cells.
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