Key result
Duck enteritis virus expresses pUL38 from 8 hours post-infection, ultimately incorporating it into virions.
Why the study?
Knowledge of the intracellular location of DEV pUL38 protein was lacking and could provide insights into its function.
DEV pUL38 mapping is preliminary; leaves open its functional role and antiviral potential in avian herpesviruses.
Knowledge of the intracellular location of a protein can provide useful insights into its function. Bioinformatic studies have predicted that the DEV pUL38 mainly targets the cytoplasm and nucleus. In this study, we obtained anti-pUL38 polyclonal sera. These antibodies were functional in western blotting and immunofluorescence in DEV-infected duck embryo fibroblasts (DEFs). pUL38 was expressed as a 51-kDa protein from 8 h post-infection onward, initially showing a diffuse distribution throughout the cytoplasm, and later in the nucleus. Furthermore, pUL38 was found in purified virus. These results provide the first evidence of the kinetics of expression and intracellular localization of DEV pUL38.
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Xiang et al. (2010) studied Duck enteritis virus (DEV) infection. Duck enteritis virus (DEV) infection vs. Mock-infected cells was evaluated on Kinetics of expression and intracellular localization of pUL38. Duck enteritis virus pUL38 is expressed as a 51-kDa protein from 8 hours post-infection onward, initially localizing in the cytoplasm and later in the nucleus, and is incorporated into virions.
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