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September 15, 2026Applied Food ResearchOpen Access

Unveiling the bioactive potential of Syzygium aromaticum bud ethanolic extract for food preservation: GC-MS, antioxidant, antibacterial, and FESEM analysis

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Authors

SASharmin AhmedITIshrat Jahan TonniNSNusrat Tabassum Shristy

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Overview

In vitro study demonstrates potent antioxidant and broad-spectrum antimicrobial efficacy of clove bud extract against foodborne pathogens, highlighting its value as a natural food preservative.

Key Points

  • To characterize the phytochemical composition and evaluate the antioxidant, antibacterial, and ultrastructural biocidal mechanisms of Syzygium aromaticum bud ethanolic extract for food preservation.
  • Fractionated clove bud ethanolic extracts across organic solvents and identified bioactives using gas chromatography-mass spectrometry (GC-MS).
  • Quantified antioxidant capacity through DPPH, ABTS, and ferric reducing antioxidant power (FRAP) assays.
  • Evaluated antimicrobial spectrum against foodborne pathogens via inhibition zone measurements, MIC, MBC, and field emission scanning electron microscopy (FESEM).
  • Ethanolic extract exhibited antioxidant capacity with IC₅₀ values of 10.42 ± 0.07 µg/mL (DPPH) and 7.62 ± 0.12 µg/mL (ABTS), plus a FRAP value of 10.16 ± 0.15 mmol Fe²⁺ eq/g.
  • Extract demonstrated potent antimicrobial activity against Salmonella typhimurium (MIC 1.56 mg/mL, MBC 3.12 mg/mL), Bacillus cereus (inhibition zone 28.33 ± 0.58 mm), and Candida albicans (50.33 ± 0.58 mm at 300 mg/mL).
  • Ultrastructural FESEM imaging revealed progressive cellular wall damage in Staphylococcus aureus, Escherichia coli, and Candida albicans following 24-hour exposure at 6.25 mg/mL.

Cite This Study

Ahmed et al. (2026) studied this question.

synapsesocial.com/papers/6aa9132f9013453be30a0efehttps://doi.org/10.1016/j.afres.2026.102603
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