Key result
Sapovirus associates with ~57% of pediatric acute gastroenteritis outbreaks, driven by the GI.6 genotype.
Why the study?
Sapovirus infection is increasing globally and several outbreaks have been observed among children in Zhejiang province, prompting analysis of Sapovirus genotypes from these events.
Observational (n=105)
GI.6 is the predominant Sapovirus genotype in recent outbreaks among children in Zhejiang province, China.
Alerts clinicians to Sapovirus GI.6 in pediatric outbreaks; leaves open broader applicability pending prospective studies.
BACKGROUND: Sapovirus (SaV) infection is increasing globally. Concurrently, several SaV-outbreaks were observed in children of Zhejiang province, China, in recent years, In this study, the genotypes of Sapovirus from seven outbreaks in the Zhejiang province were analysed. METHODS: A total of 105 faecal samples were collected from children aged between 4 and 17 years from the Zhejiang Provincial Center for Disease Control and Prevention between October 2021 and February 2023. Genotypes were processed using reverse transcription polymerase chain reaction and Sanger sequencing, while next-generation sequencing was used to generate a complete viral genome. Deduced amino acid sequences were analysed to detect VP1 gene mutations. RESULTS: In total, 60 SaV-positive patients were detected at a 57.14% (60/105) positivity rate. Positive rates in the seven outbreaks were: 22.22% (2/9), 15.00% (3/20), 93.10% (27/29), 84.21% (16/19), 28.57% (2/7), 53.33% (8/15) and 33.33% (2/6), respectively. Four genotypes were identified in the seven outbreaks, of which, GI.1 accounted for 14.29% (1/7), GI.2 accounted for 14.29% (1/7), GI.6 and GII.5 accounted for 14.29% (1/7), and GI.6 accounted for 57.14% (4/7). All patients were children and outbreaks predominantly occurred in primary schools and during cold seasons. Additionally, the complete sequence from the GI.6 outbreak strain showed high homology (identity: 99.99%) with few common substitutions (Y300S, N302S and L8M) in VP1 protein. CONCLUSIONS: SaV genotype diversity was observed in the seven outbreaks, with GI.6 being the main SaV genotype in Zhejiang province. It demonstrated high homology and may provide a platform for SaV prevention and control measures.
No takes yet. Share an insight, caveat, or question.
Su et al. (2023) conducted an observational in Acute gastroenteritis (Sapovirus outbreaks) (n=105). Sapovirus outbreak was evaluated on Sapovirus positivity rate and genotype distribution. Sapovirus was detected at a 57.14% positivity rate across seven acute gastroenteritis outbreaks in children, with the GI.6 genotype being the dominant strain responsible for 57.14% of the outbreaks.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: