In vitro experimental study demonstrates superior shoot regeneration from cotyledon node explants in sunflower, indicating an efficient protocol for micropropagation.
Key Points
To establish an efficient, reproducible in vitro organogenesis protocol for the commercial sunflower hybrid 'Sun 2239 CL' using cotyledon and root node explants.
Cultured cotyledon node and root node explants of sunflower hybrid 'Sun 2239 CL' on Murashige and Skoog (MS) medium.
Tested various concentrations of BAP (0.00, 0.25, 0.50, 0.75, and 1.00 mg/L) combined with 0.10 mg/L NAA to evaluate shoot and callus regeneration.
Cotyledon node explants achieved maximum shoot regeneration of 3.0 shoots per explant at 0.25 mg/L BAP plus 0.10 mg/L NAA, with regenerated shoot lengths ranging from 3.33 cm to 11.00 cm.
Root node explants formed no adventitious shoots but produced callus, reaching peak callus weight of 1.60 g at 1.00 mg/L BAP plus 0.10 mg/L NAA.