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September 15, 2026FishesOpen Access

Tissue-Specific Multielement Distribution in Freshwater Fish from Vrana Lake, Croatia: Insights from Advanced Multivariate Classification Techniques

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Authors

ZKZorana Kljaković‐GašpićASAnkica SekovanićTOTatjana Orct

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Overview

Observational study reveals tissue-specific accumulation of 24 elements in freshwater fish, indicating organ physiology drives elemental profiles more than species or size.

Key Points

  • To characterize the multielement distribution of 24 metal(loid)s across diverse tissues in freshwater fish and evaluate the influence of biological and environmental factors.
  • Quantified concentrations of 24 metal(loid)s in muscle, liver, gills, and gonads of dominant freshwater fish using inductively coupled plasma mass spectrometry (ICP-MS).
  • Evaluated the effects of tissue type, fish species, body size, and season using classical statistical tests, principal component analysis (PCA), and the Neural Gas (NG) machine learning algorithm.
  • Tissue type was the primary determinant of elemental profiles, with the highest concentrations occurring in the liver and gills, intermediate levels in gonads, and lowest, most uniform levels in muscle.
  • Species and size-related variations were minimal or tissue-dependent, while PCA and Neural Gas algorithms effectively resolved tissue-specific profiles and complex within-tissue heterogeneity.

Cite This Study

Kljaković‐Gašpić et al. (2026) studied this question.

synapsesocial.com/papers/6aa913e49013453be30a2214https://doi.org/10.3390/fishes11090541
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