Laboratory protocol demonstrates transcardiac perfusion and fixation in mice, highlighting optimal brain preservation for immunostaining and hybridization.
Transcardiac perfusion with saline followed by 4% paraformaldehyde (PFA) is widely used to clear blood and preserve brain for immunostaining or in situ hybridization. PFA breaks into formaldehyde in solution, which cross-link protein and DNA molecules to preserve tissue and cell structure. Here we provide a step by step guide for performing this procedure in mouse.
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Wu et al. (2021) studied this question.
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