A modified method of intracardiac perfusion fixation for tissue preparation

In animal research, a key challenge is preserving nervous tissue due to the rapid protein degradation. This study aims to improve perfusion techniques, a crucial method for nervous tissue preservation. A total of 27 mice were randomly divided into three groups: syringe perfusion group, gravity perfusion group, and modified method perfusion group. The operation time and perfusion degree of each mouse were recorded, and pathological observations were performed. The perfusion failure rate of modified method group was lower than that of the syringe perfusion group and gravity perfusion group. When the same amount of fixative was perfused, the phosphate buffered saline perfusion time, paraformaldehyde perfusion time, and total perfusion time of the modified method perfusion group were shorter than syringe perfusion group ( p < 0.05). Compared to the conventional syringe perfusion and gravity perfusion techniques, the modified intracardiac perfusion method demonstrates a superior capacity for rapid tissue fixation with enhanced perfusion efficacy in nervous tissue. Furthermore, this modified method exhibits reduced perfusion failure rates, highlighting its potential as a superior technique for optimal tissue sample preservation.