What question did this study set out to answer?

To map single-base-resolution DNA methylation dynamics during the differentiation of mammalian intestinal stem cells into mature villus epithelial cells.

May 28, 2013Genome biologyOpen Access

DNA methylation dynamics during intestinal stem cell differentiation reveals enhancers driving gene expression in the villus

View Full Paper

Ask AI

Bookmark

Authors

LKLucas TJ KaaijHubrecht Institute for Developmental Biology and Stem Cell Research MWMarc van de WeteringOncode Institute FFFang FangRTI International

Discussion

Member takes

Implication

Epigenomic profiling reveals limited DNA methylation shifts in differentiating mouse intestinal stem cells, highlighting enhancer activation by transcription factor Tcf4.

Key Points

To map single-base-resolution DNA methylation dynamics during the differentiation of mammalian intestinal stem cells into mature villus epithelial cells.
Generated a genome-wide, single-base-resolution DNA methylation map of differentiating mouse small intestinal Lgr5+ stem cells.
Analyzed DNA methylation shifts at transcriptional start sites and distal regulatory regions, integrating Tcf4 transcription factor binding data.
Identified only 50 differentially methylated regions across the entire genome, with minimal methylation changes at transcriptional start sites where differentiation-associated genes were already primed.
Demonstrated that almost all identified differentially methylated regions correspond to enhancers driving gene expression in the villus, with Tcf4 binding correlating with hypo-methylation and driving region establishment.

Cite This Study

Kaaij et al. (2013) studied this question.

synapsesocial.com/papers/6aa91aa8bc3155705956c106 https://doi.org/10.1186/gb-2013-14-5-r50

View Full Paper

Ask AI

Bookmark

Implication

Epigenomic profiling reveals limited DNA methylation shifts in differentiating mouse intestinal stem cells, highlighting enhancer activation by transcription factor Tcf4.

DNA methylation dynamics during intestinal stem cell differentiation reveals enhancers driving gene expression in the villus

Discussion

Member takes

Implication

Key Points

Cite This Study

DNA methylation dynamics during intestinal stem cell differentiation reveals enhancers driving gene expression in the villus

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider