Key result
Myo10 localizes ~6% of its intracellular pool to filopodia, sometimes exceeding accessible actin binding sites.
Why the study?
Although Myosin 10 (Myo10)-driven filopodial dynamics have been characterized, there is no information about the numbers of Myo10 in filopodia.
Quantification of Myo10 in filopodia reveals that hundreds of molecules pack into these structures, sometimes exceeding accessible actin binding sites, providing insight into filopodial molecular stoichiometries.
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Should not yet inform clinical models of cell migration; leaves open stoichiometric mechanisms in filopodia.
Shangguan et al. (2023) studied this question. HaloTag-labeled Myo10 quantitation was evaluated on Myo10 abundance in filopodia. About 6% of total intracellular Myo10 localizes to filopodia, with hundreds of Myo10 found in a typical filopodium, sometimes exceeding accessible binding sites on the actin filament bundle.
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