The aim of this work was to genotype Vibrio cholerae strains, based on 9 INDEL-markers. We identified 26 genotypes in 223 strains studied. Based on the cluster analysis, all genotypes were grouped into 8 clusters. A geographic attachment to specific regions was characteristic for some of the clusters. Toxigenic strains formed a separate cluster and have genotypes different from atoxigenic strains. All strains V cholerae O1 Eltor, isolated from 1929 to 2014, had an identical genotype, different from the genotypes of V. cholerae O1 classical and V. cholerae O139. A loss of cholera toxin gene was shown to fail to give rise in the alteration of INDEL-genotype. This allows us recommend this genotyping method for the identification strains that had a cholera toxin gene in the past. Under annual isolation of strains of V. cholerae from environmental objects, their population was established to be particular due to a high level of genetic diversity. This makes it possible to propose the use of the diversity index as one of the criteria for the assessment of epidemiological risk.
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Vodop’Yanov et al. (2017) studied this question.
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