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This study aimed to validate a comprehensive diagnostic protocol based on real-time PCR for the rapid detection and identification of Bordetella pertussis , Bordetella parapertussis , and Bordetella holmesii , as well as its implementation in the diagnostic routine of a reference children’s hospital. The new algorithm included a triplex quantitative PCR (qPCR) targeting IS 481 gene (in B. pertussis , B. holmesii , and some Bordetella bronchiseptica strains), pIS 1001 ( B. parapertussis -specific) and rnase P as the human internal control.
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Valero et al. (2018) studied this question.
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