Key result
Low-insulin-high-glucose medium prolongs relaxation and slows intracellular Ca2+ decay in rat myocytes within one day.
Why the study?
Abnormal diastole with prolonged relaxation occurs in ventricular myocytes shortly after diabetes induction, but the roles of insulin and glucose in this dysfunction are unclear.
Does a low-insulin-high-glucose environment induce abnormal relaxation in cultured adult rat ventricular myocytes?
Population
Cultured adult rat ventricular myocytes
Comparison
Low-insulin-high-glucose medium vs normal medium
Design
Serum-free myocyte culture study with mechanical and Ca2+ transient evaluation
Follow-up
1-4 days
Authors
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Offers a rapid cellular model for diabetic cardiomyopathy; leaves open translation to human disease and in vivo settings.
Does a low-insulin-high-glucose environment induce abnormal relaxation in cultured adult rat ventricular myocytes?
A low-insulin, high-glucose environment rapidly induces abnormal relaxation and impaired calcium handling in isolated ventricular myocytes, mimicking diabetic cardiomyopathy.
Davidoff et al. (1997) studied Abnormal diastole associated with diabetes. Low-insulin-high-glucose (LIHG) medium vs. Normal medium was evaluated on Indexes of contraction and relaxation (relengthening) and intracellular Ca2+ transients. Culturing adult rat ventricular myocytes in a low-insulin-high-glucose medium prolonged relaxation and slowed intracellular Ca2+ transient decay rates within 1 day.
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