Key result
HAdV26 infection of human epithelial cells relies on caveolin-1 and partially on dynamin-2.
Why the study?
Endocytosis and intracellular trafficking of HAdV26, extensively studied as a vaccine vector, had not been described.
HAdV26 cell entry is mediated by caveolin-1 and partially dynamin-2, which may influence the immunogenicity and antigen presentation of HAdV26-based vaccine vectors.
In order to fulfill its role as a vector, adenovirus needs to successfully deliver its DNA genome to the host nucleus, a process highly influenced by adenovirus intracellular translocation. Thus, cell entry pathway and intracellular trafficking determine functionality of human adenovirus-based vectors. Endocytosis of HAdV26, currently extensively studied as a vaccine vector, has not been described so far. We present here that HAdV26 infection of human epithelial cells with high expression of αvβ3 integrin, one of the putative HAdV26 receptors, is caveolin-1- and partially dynamin-2-dependent. Since caveolin containing domains provide a unique environment for specific signaling events and participate in inflammatory signaling one can imagine that directing HAdV26 cell entry toward caveolin-1-mediate pathway might play role in immunogenicity of this virus. Therefore, our results contribute to better understanding of HAdV26 infection pathway, hence, can be helpful in explaining induction of immune response and antigen presentation by HAdV26-based vaccine vector.
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Nestić et al. (2022) studied Human Adenovirus Type 26 (HAdV26) infection. HAdV26 infection was evaluated on Cell entry pathway and intracellular trafficking. HAdV26 infection of human epithelial cells with high expression of αvβ3 integrin is dependent on caveolin-1 and partially on dynamin-2.
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