What is the clinical evidence from this study?

Study design: Other. Intervention: YFP-myosin XI-tail fusions. Primary outcome: Organelle localization and motility.

What does this research mean for the field?

Arabidopsis class XI myosin tails specifically target mobile plant cell vesicles and organelles via their coiled-coil domains without inhibiting organelle motility. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

January 1, 2007BMC Plant BiologyOpen Access

Association of six YFP-myosin XI-tail fusions with mobile plant cell organelles.

View Full Paper

Ask AI

Bookmark

Key result

YFP-myosin XI-tail fusions specifically label motile plant organelles, requiring the coiled-coil region for localization.

Population

Plant cells (Arabidopsis)

Design

Preclinical

Authors

DRDaniel ReisenUnion Bank of Switzerland MHMaureen R. HansonCornell University

Discussion

Member takes

Implication

May enable organelle tracking in plant cells; leaves open validation across species and conditions.

Structured PICO

Population

Plant cells (Arabidopsis)

Intervention

Transient expression of 6 YFP-myosin XI-tail fusions (myosin XI-5, XI-6, XI-8, XI-15, XI-16, XI-17)

Outcome

Organelle localization and motilitysurrogate

Six Arabidopsis myosin XI tails specifically label motile vesicles and organelles when expressed as YFP fusions in plant cells, providing a tool for future organelle identification.

Limitations

Transient expression may not reflect endogenous protein behavior
YFP and GFP signals can be difficult to separate due to overlapping excitation peaks
Potential for dominant negative effects or artifacts from overexpression

Cite This Study

Reisen et al. (2007) studied this question. YFP-myosin XI-tail fusions was evaluated on Organelle localization and motility. Transient expression of six YFP-myosin XI-tail fusions in plant cells resulted in specific labeling of motile vesicles and organelles, with the coiled-coil region being required for specific localization.

synapsesocial.com/papers/6aa933ad86badb2eb40e00e3 https://doi.org/10.1186/1471-2229-7-6

View Full Paper

Ask AI

Bookmark

Association of six YFP-myosin XI-tail fusions with mobile plant cell organelles.

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Association of six YFP-myosin XI-tail fusions with mobile plant cell organelles.

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider