What question did this study set out to answer?

To establish an integrative single-cell and molecular profiling pipeline to characterize the immunological effects and biocompatibility of pristine versus amino-functionalized graphene oxide.

October 18, 2017Nature CommunicationsOpen Access

Single-cell mass cytometry and transcriptome profiling reveal the impact of graphene on human immune cells

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To establish an integrative single-cell and molecular profiling pipeline to characterize the immunological effects and biocompatibility of pristine versus amino-functionalized graphene oxide.
Evaluated the effects of graphene oxide (GO) and amino-functionalized graphene oxide (GONH2) across 15 distinct human immune cell populations.
Interrogated 30 surface and intracellular markers at single-cell resolution using mass cytometry.
Integrated single-cell mass cytometry with genome-wide transcriptome profiling to evaluate cellular metabolism and activation pathways.
Amine group functionalization markedly reduced GO-induced metabolic perturbations and enhanced cellular biocompatibility across immune subsets.
Exposure to GONH2 selectively shifted T-cell and monocyte activation toward a pro-inflammatory T helper-1 (Th1) and M1-like immune response.

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Implication

In vitro multi-omic profiling reveals that amine functionalization improves graphene biocompatibility in human immune cells, highlighting strategies for safe nanomedicine design.

Cite This Study

Orecchioni et al. (2017) studied this question.

synapsesocial.com/papers/6aa93d4cd6d58a1cc10c081d https://doi.org/10.1038/s41467-017-01015-3

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Implication

In vitro multi-omic profiling reveals that amine functionalization improves graphene biocompatibility in human immune cells, highlighting strategies for safe nanomedicine design.

Single-cell mass cytometry and transcriptome profiling reveal the impact of graphene on human immune cells

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Discussion

Implication

Cite This Study

Single-cell mass cytometry and transcriptome profiling reveal the impact of graphene on human immune cells

Key Points

Discussion

Implication

Cite This Study

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