Key result
Sac1 phosphatase and PI4P promote Tombusvirus replication by facilitating replicase assembly and recruiting host factors.
Why the study?
Replication of positive-strand RNA viruses depends on the recruitment of host components into viral replication compartments.
Sac1 and PI(4)P play critical roles in promoting TBSV replication by facilitating the assembly of viral replicase complexes and enriching specific lipids within subverted host membranes.
May enable host-targeted antivirals against positive-strand RNA viruses; leaves open validation in human pathogens.
Replication of positive-strand RNA viruses depends on recruitment of host components into viral replication compartments or organelles. Using TBSV, we uncovered the critical roles of Sac1 PI(4)P phosphatase and its substrate, PI(4)P phosphoinositide, in promoting viral replication. Both Sac1 and PI(4)P are recruited to the site of viral replication to facilitate the assembly of the viral replicase complexes, which perform viral RNA replication. We found that Sac1 affects the recruitment of other host factors and enrichment of phosphatidylethanolamine and sterol lipids within the subverted host membranes to promote optimal viral replication. In summary, this work demonstrates the novel functions of Sac1 and PI(4)P in TBSV replication in the model host yeast and in plants.
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Sasvári et al. (2020) studied Tombusvirus replication. Sac1 PI(4)P phosphatase and PI(4)P phosphoinositide was evaluated on Viral replication. Sac1 PI(4)P phosphatase and PI(4)P phosphoinositide promote optimal Tombusvirus replication by facilitating the assembly of viral replicase complexes and recruiting host factors and lipids.
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