What does this research mean for the field?

Differential expression levels of integrin α6 (ITGA6) enable the selective, transgene-free identification and isolation of pure atrial and ventricular cardiomyocytes across mouse developmental stages. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

November 30, 2015PLoS ONEOpen Access

Differential Expression Levels of Integrin α6 Enable the Selective Identification and Isolation of Atrial and Ventricular Cardiomyocytes

Q: What is the clinical evidence from this study?

Study design: Other. Population: Cardiomyocyte subtype identification. Intervention: Antibody-based flow cytometry sorting using Integrin α6 (ITGA6) vs. Unsorted cells. Primary outcome: Selective identification and isolation of atrial and ventricular cardiomyocytes.

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Key result

Differential ITGA6 expression enables selective isolation of highly pure atrial and ventricular mouse cardiomyocytes.

Population

Embryonic (CD1, wild-type, E11.5–E17.5), neonatal (P2), P8, and adult mouse hearts.

Design

Preclinical

Authors

AWAnne Maria WiencierzMiltenyi Biotec (Germany)MKManuel KernbachUniversity of Cologne JEJosephine EcklebeMiltenyi Biotec (Germany)

Discussion

Member takes

Implication

ITGA6 enables mouse cardiomyocyte subtype purification; leaves open validation for human research or cell therapy applications.

Structured PICO

Population

Embryonic, neonatal, and adult mouse hearts were utilized to develop a non-genetic, antibody-based approach for isolating cardiomyocyte subtypes.

Intervention

Non-genetic, antibody-based isolation procedure using differential expression levels of integrin α6 (ITGA6) and flow cytometry.

Outcome

Selective identification and isolation of atrial and ventricular cardiomyocytes.surrogate

Differential expression of integrin α6 enables the non-genetic, antibody-based selective isolation of highly pure atrial and ventricular cardiomyocytes from mouse hearts across various developmental stages.

Limitations

The two-marker sorting strategy using ERBB-2 is restricted to embryonic hearts because ERBB-2 expression decreases after birth.

Cite This Study

Wiencierz et al. (2015) studied Cardiomyocyte subtype identification. Antibody-based flow cytometry sorting using Integrin α6 (ITGA6) vs. Unsorted cells was evaluated on Selective identification and isolation of atrial and ventricular cardiomyocytes. Differential expression levels of Integrin α6 (ITGA6) enable the selective identification and isolation of highly pure atrial and ventricular cardiomyocytes from mouse hearts.

synapsesocial.com/papers/6aa93e5a11a60bccbd26450c https://doi.org/10.1371/journal.pone.0143538

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Differential Expression Levels of Integrin α6 Enable the Selective Identification and Isolation of Atrial and Ventricular Cardiomyocytes

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Differential Expression Levels of Integrin α6 Enable the Selective Identification and Isolation of Atrial and Ventricular Cardiomyocytes

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider