Key result
Intramuscular ketorolac synovial fluid levels peak at 3 hours, lagging behind serum concentrations.
Why the study?
A sensitive and selective method to quantify ketorolac in serum and synovial fluid of rheumatologic patients was needed for routine monitoring and pharmacokinetic studies.
Observational (n=20)
No
A rapid and sensitive HPLC method was developed and validated for monitoring ketorolac concentrations in serum and synovial fluid, suitable for routine clinical use and pharmacokinetic studies.
New HPLC assay enables ketorolac monitoring in synovial fluid; leaves open optimized dosing pending larger pharmacokinetic studies.
A rapid, selective and sensitive isocratic reversed-phase HPLC assay coupled with UV detection for quantifi cation of ketorolac in serum and synovial fl uid samples has been developed.Analytes were extracted on solid-phase cartridges (SPE) and chromatographic separation was achieved on a C18 column.The chromatographic peak area ratio based on UV absorbency at 313 nm was used for quantitative analysis.This HPLC method has been successfully used for routine evaluation to monitor serum and synovial fl uid concentrations in reumathologic patients affering to our institute.Thanks to its sensitivity, this HPLC/UV method is also suitable for pharmacokinetic studies.
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Franceschi et al. (2010) conducted an observational in Rheumatic diseases (n=20). Ketorolac was evaluated on Serum and synovial fluid concentrations (Cmax, Tmax, AUC). A newly developed HPLC method demonstrated that after a 30 mg IM dose, ketorolac synovial fluid concentrations peaked at 3 hours (441.38 ng/mL), compared to 1.5 hours (2152.40 ng/mL) in serum.
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