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January 1, 2003Applied and Environmental MicrobiologyOpen Access

Diversity, Frequency, and Persistence ofEscherichia coliO157 Strains from Range Cattle Environments

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Authors

DRDavid G. RenterKansas State University JSJan M. SargeantUniversity of Guelph RORichard D. OberstKansas State University

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Implication

Longitudinal surveillance demonstrates high genetic diversity and environmental persistence of Escherichia coli O157 across range cattle environments, highlighting complex transmission risks.

Key Points

Determine the genetic diversity, isolation frequency, and environmental persistence of Escherichia coli O157 strains across extensive range cattle production systems.
Conducted an 11-month surveillance study collecting 9,122 cattle fecal samples, 4,083 water source samples, and 521 wildlife fecal samples.
Identified presumptive isolates via culture and latex agglutination, confirmed intimin and Shiga toxin genes by PCR, and resolved subtypes using XbaI pulsed-field gel electrophoresis (PFGE).
PCR confirmed 90.1% of presumptive isolates, yielding 79 distinct XbaI-PFGE subtypes across 235 typeable isolates, with an average of 1.80 subtypes per positive sample.
Most subtypes (54 of 79) appeared only once, but the seven most prevalent subtypes accounted for over half of all isolates (124 of 235) and persisted for up to 10 months.
Identified seven identical subtypes in both cattle feces and water sources, including one matching subtype isolated from wild opossum (Didelphis sp.) feces.

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Renter et al. (2003) studied this question.

synapsesocial.com/papers/6aa9418a3c7019b2508aaa6b https://doi.org/10.1128/aem.69.1.542-547.2003

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Implication

Longitudinal surveillance demonstrates high genetic diversity and environmental persistence of Escherichia coli O157 across range cattle environments, highlighting complex transmission risks.

Diversity, Frequency, and Persistence ofEscherichia coliO157 Strains from Range Cattle Environments

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Implication

Key Points

Cite This Study

Diversity, Frequency, and Persistence ofEscherichia coliO157 Strains from Range Cattle Environments

Discussion

Member takes

Implication

Key Points

Cite This Study

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