Residual ivermectin in cattle dung was determined in order to measure its rate of excretion and persistence under field conditions. The drug was extracted into methanol and subsequently determined by reversed-phase high performance liquid chromatography using a 300 × 3.9 mm Bondclone C18 column, 47:33:20 acetonitrile/methanol/water as the mobile phase and UV detection at 245 nm. The determination limit for 5 g of sample was 0.020 mg kg−1. HPLC analyses for aminoacids in the dung were suggestive of changes in their contents with time, which may account for the differential behaviour of insects attracted by the dung.
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Bernal et al. (1994) studied this question.
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