MiR-199a-3p suppresses proliferation and invasion of prostate cancer cells by targeting Smad1

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// Feng Qu 1, 2, * , Jinyu Zheng 3, * , Weidong Gan 1, 2, * , Huibo Lian 1, 2 , Hua He 4 , Wuping Li 4, 5 , Tian Yuan 4 , Yaling Yang 4 , Xiaogong Li 1 , Changwei Ji 1, 2 , Xiang Yan 1, 2 , Linfeng Xu 1, 2 and Hongqian Guo 1, 2 1 Department of Urology, The Affiliated Nanjing Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, 210008, China 2 Institute of Urology, Nanjing University, Nanjing, Jiangsu, 210093, China 3 Department of Pathology, The Affiliated Nanjing Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, 210008, China 4 Department of Hematopathology, Division of Pathology and Laboratory Medicine, University of Texas MD Anderson Cancer Center, Houston, Texas, 77030, USA 5 Department of Lymphoma, Jiangxi Cancer Hospital, Nanchang, Jiangxi, 330029, China * These authors contributed equally to this work Correspondence to: Feng Qu, email: qufeng01@gmail.com Hongqian Guo, email: dr.guohongqian@gmail.com Keywords: MiR-199a-3p, Smad1, prostate cancer, proliferation, invasion Received: February 10, 2017 Accepted: March 10, 2017 Published: April 18, 2017 ABSTRACT Objectives: This study was intended to analyze effects of miR-199a-3p and Smad1 on proliferation, migration and invasion of prostate cancer (PCa) cells. Results: MiR-199a-3p was significantly decreased in PCa tissues in comparison to that in adjacent normal tissues ( P < 0.05). Over-expressed miR-199a-3p markedly suppressed proliferation and invasion of PCa cells ( P < 0.05). MiR-199a-3p was negatively correlated with Smad1 expression, and overexpression of Smad1 could antagonize the effects of miR-199a-3p on PCa cells. Materials and methods: The PCa tissues and their adjacent normal tissues were collected from 54 PCa patients. Expressions of miR-199a-3p and Smad1 mRNA in tissues and cells were evaluated with real-time quantitative polymerase chain reaction (RT-qPCR), and immunohistochemistry assay was used to detect Smad1 protein expressions. The target relationship between miR-199a-3p and Smad1 was assessed by luciferase reporter assay. The PCa cell lines ( i.e. PC-3 cells) were transfected with miR-199a-3p mimics and Smad1-cDNA. MTT and Transwell assays were applied to detect proliferative, migratory and invasive abilities of PCa cells. Conclusions: MiR-199a-3p suppressed proliferation and invasion of PCa cells by targeting Smad1.