Electrical fusion of protoplasts was used to produce somatic hybrids between Mexican lime (Citrus aurantifolia Swingle) and Java feroniella (Feroniella lucida (Sceff.) Swingle) or tabog (Swinglea glutinosa (Blanco) Men.). The fusion efficiency (rates of formation of binuclear heterokaryons) were around 15%. The fusion products were cultured and embryoids were differentiated from them. The embryoids were transferred to a medium for plantlet formation and grew into plantlets. The rDNA analysis confirmed that the differentiated plantlets from these two combinations have genomes from both parents. The results confirmed that our electrical fusion system is an effective method for producing somatic hybrids of citrus and its sexually incompatible wild relatives.
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Takayanagi et al. (1992) studied this question.
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