Key result
Combined chronic stress and diabetes exacerbates cardiac matrix remodeling in rats, increasing gelatinase activity ~63%.
Why the study?
Extracellular matrix remodelling under chronic stress associated or not with diabetes remains unknown.
The combination of diabetes and chronic mild stress exacerbates extracellular matrix remodeling in the rat heart, highlighting a potential mechanism for increased cardiovascular risk in patients with both conditions.
Answers are grounded in this paper and cite their sources.
May indicate added cardiac risk in comorbid diabetes and stress; hypothesis-generating for human ECM remodeling.
The coexistence of depression and diabetes is a serious challenge in cardiovascular disease. However, extracellular matrix (ECM) under stress model associated or not with diabetes remains unknown. This study aims to investigate the involvement of diabetes and unpredictable chronic mild stress (UCMS) on ECM remodelling. Rats were exposed to UCMS, diabetes or combined treatment. mRNA expression of matrix metalloproteinases (MMP-2 and MMP-9), plasminogen activator (t-PA) and inhibitor (PAI-1) was examined by Q-RT-PCR. ECM was determined by ELISA. MMP-2 and MMP-9 mRNA was lower in diabetes (p< 0.05). UCMS increased MMP-2 and MMP-9 compared to control and diabetic group. Compared to the control and diabetes groups, MMP-2 and MMP-9 mRNA was significantly increased in the combined treatment group. UCMS increased MMP-2 expression in the diabetic group (p <0.01). Compared to control, gelatinase activity was higher in diabetes and UCMS (p <0.05). Combined diabetes and UCMS significantly increased gelatinase activity compared to T2D and UCMS groups. Collagen, hydroxyproline and fibronectin content were significantly increased in diabetes and combined groups. UCMS combined with diabetes exacerbated diabetes-induced MMPs and fibronectin deposition. In conclusion, comorbidity between diabetes and UCMS may exacerbate ECM remodelling. These findings will be useful in understanding diabetes-induced cardiovascular disease.
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Dab et al. (2023) studied Diabetes and unpredictable chronic mild stress (n=30). Combined unpredictable chronic mild stress (UCMS) and diabetes vs. Control, diabetes alone, and UCMS alone was evaluated on Gelatinase activity in heart tissue (63% increase, p=<0.01). The combination of unpredictable chronic mild stress and diabetes significantly exacerbated extracellular matrix remodeling in the rat heart, increasing gelatinase activity by 63% compared to controls.
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