Isolation and Regeneration of Protoplasts of the Moss Physcomitrella patens: Figure 1.

INTRODUCTION This protocol describes how to isolate individual protoplasts from young gametophyte tissue of the moss Physcomitrella patens and how to regenerate them into plants if desired. Protonemal tissue is grown on cellophane-overlay plates, harvested, and treated with 0.5% Driselase to produce protoplasts. After filtration and washing, the protoplasts are regenerated on solid medium.