Genomic Characterization of HLJDZD55: The First L1B PRRSV in China

Figures

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  Figure 1. Genotyping of newly emerged PRRSV in China. (a) Phylogenetic tree constructed based on the ORF5 gene of HLJDZD55 and reference PRRSV strains from each lineage. The red branches represent the L1B strains, the yellow branches represent the L1A and L1C–L1J strains, and the blue branches represent the L2–L11 strains. HLJDZD55 was classified as belonging to the L1B branch and is labeled with ●. (b) Alignment of the deduced amino acid sequence based on the Nsp2 gene. The positions marked in the figure represent positions of the Nsp2 amino acid sequence and refer to the position of ATCC VR2332. Gray indicates the NADC34-like PRRSV 100 aa characteristic ontinuous deletion. Compared with the VR2332 Nsp2 gene, the HLJDZD55 and L1B strains have a discontinuous deletion of 131 amino acids (111 + 1 + 19), which are marked in yellow. HLJDZD55 is labeled in red.

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  Figure 2. Recombination analysis of strain HLJDZD55. (a) Comparison of the similarity of HLJDZD55 and potential parental viruses using SimPlot. Recombination breakpoints are shown as black dotted lines. The background color of the major parental region (TJZH-1607) is white, whereas that of the minor parental region (Minnesota 14) is gray. TJZH-1607 and Minnesota 14 are shown in red and blue, respectively. The phylogenies of the major parents (b) and the minor parents (c) are shown as the similarity plots. The major parental groups (TJZH-1607 and L1C) are shown in red, while the minor parental groups (Minnesota 14 and L1B) are shown in blue; the other lineages are shown in black. HLJDZD55 is labeled with ●.