Plant material and extract preparationLeaves and fruits of a single individual of A. colubrina were collected at Catimbau National Park (Buíque, Pernambuco, Brazil) at September 2010 and January, April and June 2011.The rainfall index for this months were 75 mm, 65 mm, 162 mm and 73 mm, respectively (as provided by Agronomic Institute of Pernambuco; IPA/PE).The voucher specimen (IPA 84.039) is deposited at IPA/PE.The plant material was dried at 45°C and after 3 days milled to a fine powder in a Macsalab Mill (Model 200 LAB, Eriez ® , Bramley), and stored at room temperature in closed containers in the dark until used.
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Araújo et al. (2017) studied this question.
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