Key result
Novel padlock probe assay detects rotavirus with high PCR concordance and a 1,000-copy limit.
A novel padlock probe and rolling circle amplification assay provides a sensitive and variation-tolerant method for detecting rotavirus in clinical samples.
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May support alternative rotavirus detection; leaves open clinical adoption pending larger validation studies.
Rotavirus infections are one of the most common reasons for hospitalizations due to gastrointestinal diseases. Rotavirus is often diagnosed by latex agglutination assay, chromatography immunoassay, or by electron microscopy, which are all quite insensitive. Reverse transcription polymerase chain reaction, on the other hand, is very sensitive to variations at the genomic level. We developed a novel assay based on a set of 58 different padlock probes with a detection limit of 1,000 copies. Twenty-two patient samples were analyzed and the assay showed high concordance with a PCR-based assay. In summary, we present a new assay for sensitive and variation tolerant detection of rotavirus.
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Mezger et al. (2014) studied Rotavirus infection (n=22). Padlock probes and rolling circle amplification assay vs. RT-PCR was evaluated on Detection of rotavirus and concordance with PCR. A novel assay using padlock probes and rolling circle amplification successfully detected rotavirus in clinical samples with a detection limit of 1,000 copies and high concordance with PCR.
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