What does this research mean for the field?

Outside-to-inside (reverse) signaling through membrane TNF-alpha induces the expression of the adhesion molecule E-selectin (CD62E) on activated human CD4+ T cells, demonstrating its role as a bipolar signaling molecule in local inflammation. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.ESTABLISHES_NEW_DIRECTION.

What question did this study set out to answer?

To investigate outside-to-inside (reverse) signaling mediated by membrane TNF-alpha and assess whether it induces the cell adhesion molecule E-selectin in T cells.

January 1, 2001The Journal of Immunology

Outside-to-Inside Signal Through the Membrane TNF-α Induces E-Selectin (CD62E) Expression on Activated Human CD4+ T Cells

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Authors

SHShin‐ichi HarashimaKagawa University THTakahiko HoriuchiARCH Development Corporation NHNobuaki HattaEhime University

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Implication

In vitro study demonstrates that reverse signaling through membrane TNF-alpha induces E-selectin expression on activated T cells, indicating a novel bipolar role in local inflammation.

Key Points

To investigate outside-to-inside (reverse) signaling mediated by membrane TNF-alpha and assess whether it induces the cell adhesion molecule E-selectin in T cells.
Stimulated membrane TNF-alpha on MT-2 T cells and PHA-activated normal human CD4+ T cells using an anti-TNF-alpha antibody.
Expressed wild-type or cleavage-resistant mutant (R78T/S79T) membrane TNF-alpha in Jurkat and HeLa cells, activating them with antibodies or co-culture with TNF receptor-expressing cells.
Evaluated E-selectin surface expression kinetics and analyzed transcriptional regulation using Northern blot and RT-PCR.
Antibody-mediated cross-linking of membrane TNF-alpha induced E-selectin surface expression that peaked between 12 and 24 hours and disappeared by 48 hours.
Direct cell-to-cell contact between membrane TNF-alpha-expressing Jurkat cells and TNF receptor-bearing HeLa cells successfully upregulated E-selectin.
Northern blot and RT-PCR analyses confirmed that reverse signaling through membrane TNF-alpha upregulates E-selectin at the transcriptional level.

Cite This Study

Harashima et al. (2001) studied this question.

synapsesocial.com/papers/6aa95d53398b99bbe68cc6ba https://doi.org/10.4049/jimmunol.166.1.130

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Implication

In vitro study demonstrates that reverse signaling through membrane TNF-alpha induces E-selectin expression on activated T cells, indicating a novel bipolar role in local inflammation.

Outside-to-Inside Signal Through the Membrane TNF-α Induces E-Selectin (CD62E) Expression on Activated Human CD4+ T Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Outside-to-Inside Signal Through the Membrane TNF-α Induces E-Selectin (CD62E) Expression on Activated Human CD4+ T Cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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