Laboratory study reveals six polymorphic microsatellite loci in Pleurotus eryngii and P. ferulae, highlighting a viable genomic enrichment technique for fungi with rare repeat markers.
Six polymorphic microsatellite loci were developed for the closed basidiomycete species Pleurotus eryngii and P. ferulae using an enriched genomic library. The number of alleles per locus varied between two and eight and the expected heterozygosity ranged from 0.30 to 0.72 in P. eryngii and from 0.20 to 0.73 in P. ferulae. This enrichment technique is useful in those species where microsatellite loci are rare in the genome.
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Rosa et al. (2004) studied this question.
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