Differential recoveries from faecal cultures of larvae of some gastro-intestinal nematodes of cattle

Faecal cultures were established using bovine faeces containing known numbers of eggs from either Oesophagostomum radiatum, Haemonchus placei, Cooperia pectinata or a mixture of all three. A substantially greater percentage of larvae was recovered from cultures of O. radiatum and C. pectinata than was recovered from cultures of H. placei. The same pattern was observed in mixed cultures although yields of larvae from all species in mixed cultures were significantly reduced (p less than 0.001). The lower recovery of H. placei was not associated with a lower viability of H. placei eggs. Of the three different methods of harvesting larvae, the jar and mesh recovery technique was the least effective and significantly fewer larvae were recovered with this technique than with the Baermann and inversion techniques (p less than 0.05). The results are discussed with reference to the use of faecal culture and larval differentiation in the diagnosis of mixed species nematode infections of cattle.