A method is given for the determination of glycoladehyde by oxidation with perchloric acid and reaction with 2,4-dinitrophenylhydrazine. The resulting compound is extracted with benzene, made alkaline with sodium ethoxide and determined spectrophotometrically at 560 mµ. The compound is stable for at least 1 hour and has a large extinction coefficient (4·17 × 104 litres per mole per cm).
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Basson et al. (1967) studied this question.