SC2: A Novel Process for Manufacturing Multipurpose High‐Density Chemical Microarrays

Various formats of miniaturized arrays of cellulose‐bound synthetic probe molecules to suit a broad range of technical and assay configurations have been produced by a novel process which we call spotting compound‐support conjugates, SC2. This process involves parallel chemical assembly of the probe molecules by SPOT‐synthesis on a special cellulose membrane, post‐synthesis separation of the tethered compound spots, solubilization of the cellulose matrix and spotting of the soluble cellulose‐probe conjugates onto various types of planar surfaces. The performance of such arrays are demonstrated using a set of synthetic peptide analogues, assayed with a monoclonal antibody, as well as utilizing a small molecule library of diketopiperazines assayed with fluorescently labelled calmodulin.