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We report the development of a highly sensitive and selective colorimetric detection method for glutathione (GSH) over cysteine (Cys) or homocysteine (Hcy) using gold nanoparticles (AuNPs). The detection scheme is based on the quasi-stable state of AuNPs in the presence of sodium nitrate and GSH. The significant differences in interparticle forces and the assembly process of AuNPs enable the discrimination of GSH over Cys and Hcy. The presence of GSH can be detected by the naked eye and its concentration determined by UV/Vis spectrometry.
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Hu et al. (2013) studied this question.
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